Just remember. A fretted instrument is imperfect by physics. You can get it very close. But it will never have 100% perfect intonation on 100% of the strings on 100% of the frets. You can get it very close that the ear won't hear it. But a tuner will.It does to me , I like my guitars set up a certain way, And I want intonation set perfect, I won't settle for anything else but.
Kinda like, I play more up by the nut (cowboy chords) and not near as much around the 12th fret or above. So any tweaking I do is to try and insure those strings are as close as possible.I always intonate as perfectly as possible from the 12th fret when I do any string changes/setups. Problem is, there is no such thing as perfect intonation up and down the fretboard with traditional straight frets. Just not possible. So sometimes you have to adjust slightly "off" to ensure the most number of positions are as close to intonated as possible.
I try to set it as close as one can. Past that if I cannot hear it on the higher frets then it is irrelevant.

Yeah somewhat. An open G or D HAS to sound right. But also, if I am in upper register the notes still have to sound on but it is way harder to hear without checking with a tuner.In my feeble mind, I feel it also makes a difference how and what you play. I don’t play lead. Jethro does. I play a lot of chords. Betting he plays a lot of single notes. Bending notes. Thinking that intonation not quite right is going to rear its ugly head with chords and maybe not quite as much with notes?
You know that part of the middle guitar solo in “Dream On” by Aerosmith, where Joe slides up to this high note? The intonation of that guitar was off, and that note is like nails on a chalkboard for me.In my feeble mind, I feel it also makes a difference how and what you play. I don’t play lead. Jethro does. I play a lot of chords. Betting he plays a lot of single notes. Bending notes. Thinking that intonation not quite right is going to rear its ugly head with chords and maybe not quite as much with notes?
Intonation off or just plain out of tune. The way some players attack the strings, they have a hard time just keeping them in tune.You know that part of the middle guitar solo in “Dream On” by Aerosmith, where Joe slides up to this high note? The intonation of that guitar was off, and that note is like nails on a chalkboard for me.
It matters on notes, too.
I have heard of that. Magnetic pull on the strings if pup is too close.Believe it or not, I once had an issue getting the low E string to intonate properly at the 12th fret on my 2006 Strat. In a state of trying anything to see what works, I lowered the bridge pickup on the bass side down a bit and was able to get the intonation correct.
I had the bridge pickup very close to the strings in an effort to get more "fatness" and output from the bridge pickup. Being a traditional Strat single-coil pickup where each pole piece is a magnet, I'm hypothesizing that the magnetic pull was strong enough to interfere with the 12th fret intonation.
I have heard of that. Magnetic pull on the strings if pup is too close.
Something I’ve read and have tried. The G and B string seem to be problem causers. Perfectly in tune open. Then fret the darn things an of course they go sharp. Something about the human ear can detect sharp easier than flat. The suggestion was to tune the two a cent or two flat. Most can’t tell… but when you fret them since you’re starting a little flat they go neutral. I have tried that. Honestly can’t tell how much if any difference it made.No you can't tune the guitar perfectly, that 's the nature of the beast. My guitars are intonated perfect to my ears. Many things can cause intonation problems, bad strings, and the list goes on. But makes, for good discussion.
Why? Why would you allow yourself to think that perfect intonation is achievable at every fret level up or down the fretboard when it's nearly impossible?I’m a fanatic about intonation.
I do t think that at all. I’m just fanatical about getting it as tight as possible.Why would you allow yourself to think that perfect intonation is achievable at every fret level up or down the fretboard when it's nearly impossible?